Journal: RSC Advances

Article Title: Design, synthesis, and mechanistic evaluation of novel pyrazole/thiazole chalcone hybrids as dual tubulin polymerization and COX-2 inhibitors with potent antiproliferative activity

doi: 10.1039/d6ra03557d

Figure Lengend Snippet: Selected thiazole/chalcone-based tubulin polymerization inhibitors (I–VII) and combretastatin A-4.

Article Snippet: The effects of compounds 9a–o on tubulin polymerization were assessed using a fluorescence-based tubulin polymerization assay (Cytoskeleton, Inc., Cat. No. BK011P), with combretastatin A-4 as the reference inhibitor.

Techniques:

Journal: RSC Advances

Article Title: Design, synthesis, and mechanistic evaluation of novel pyrazole/thiazole chalcone hybrids as dual tubulin polymerization and COX-2 inhibitors with potent antiproliferative activity

doi: 10.1039/d6ra03557d

Figure Lengend Snippet: Tubulin polymerization inhibitory activity of compounds 9a–o and the reference inhibitor CA-4, expressed as IC 50 values (µM), as determined from a fluorescence-based polymerization assay monitored kinetically for 60 min at 37 °C. Data are presented as mean ± SEM.

Article Snippet: The effects of compounds 9a–o on tubulin polymerization were assessed using a fluorescence-based tubulin polymerization assay (Cytoskeleton, Inc., Cat. No. BK011P), with combretastatin A-4 as the reference inhibitor.

Techniques: Activity Assay, Fluorescence, Polymerization Assay

Journal: RSC Advances

Article Title: Design, synthesis, and mechanistic evaluation of novel pyrazole/thiazole chalcone hybrids as dual tubulin polymerization and COX-2 inhibitors with potent antiproliferative activity

doi: 10.1039/d6ra03557d

Figure Lengend Snippet: Superimposition of the co-crystallized (green) and redocked (brown) colchicine poses within the colchicine-binding site of tubulin (PDB ID: 4O2B).

Article Snippet: The effects of compounds 9a–o on tubulin polymerization were assessed using a fluorescence-based tubulin polymerization assay (Cytoskeleton, Inc., Cat. No. BK011P), with combretastatin A-4 as the reference inhibitor.

Techniques: Binding Assay

Journal: RSC Advances

Article Title: Design, synthesis, and mechanistic evaluation of novel pyrazole/thiazole chalcone hybrids as dual tubulin polymerization and COX-2 inhibitors with potent antiproliferative activity

doi: 10.1039/d6ra03557d

Figure Lengend Snippet: Binding interactions of compound 9l within the colchicine-binding site of tubulin (PDB ID: 4O2B): (A) 2D interaction diagram and (B) 3D binding mode.

Article Snippet: The effects of compounds 9a–o on tubulin polymerization were assessed using a fluorescence-based tubulin polymerization assay (Cytoskeleton, Inc., Cat. No. BK011P), with combretastatin A-4 as the reference inhibitor.

Techniques: Binding Assay

Journal: bioRxiv

Article Title: Capturing Translation in Action with Protein Synthesis Profiling

doi: 10.1101/2025.11.17.688896

Figure Lengend Snippet: a, Structural model of APEX2-eEF2 bound to the ribosome. The large ribosomal subunit is shown in cyan, the small subunit in green, APEX2-eEF2 in magenta, and mRNA as a black line with the red circles indicating the alkyne modification. The blue sphere marks the ∼25 nm labeling radius of APEX2. b, Expression constructs for APEX2 and APEX2-eEF2 under the TDH3 promoter. c, Western blot detection of APEX2 and APEX2-eEF2 expression. Lanes: control (empty vector), APEX2 (27 kDa), and APEX2-eEF2 (118 kDa). Tubulin (Tub) was probed as a loading control. d, Schematic of the RNA tagging workflow. Yeast cells were incubated with alkyne-phenol (30 min), followed by H₂O₂ (5 min). After quenching, total RNA was extracted, conjugated to biotin-azide via click chemistry, and enriched with streptavidin beads. Both total and enriched RNA were used for preparing Illumina sequencing libraries. e, Agarose gel analysis of total RNA from control, APEX2, and APEX2-eEF2 cells. The presence of intact 25S and 18S rRNA bands indicates high RNA quality. M, molecular weight ladder. f, Detection of alkyne-labeled RNAs by conjugation with fluorescein-azide. Total RNA from control, APEX2, and APEX2-eEF2 cells was subjected to click chemistry and analyzed by agarose gel electrophoresis. Fluorescence was detected using a Typhoon imager. g, The same gel as in F, stained with SafeStain to verify equal RNA loading. h, Quantification of fluorescein-labeled RNA signal. The bar graph shows fluorescence intensity normalized to total RNA, averaged across two independent experiments. i, Gel-shift assay of biotin-labeled RNAs incubated with anti-biotin-AF488 antibody. RNAs from control, APEX2, and APEX2-eEF2 cells were conjugated with biotin-azide, bound by antibody, and resolved on an agarose gel. Antibody-RNA complexes are indicated by the black bar. Lanes: M, molecular weight ladder; control, RNA from control cells; APEX2, RNA from APEX2-expressing cells; APEX2-eEF2, RNA from APEX2-eEF2-expressing cells; Ab, antibody only.

Article Snippet: After incubation, RNA was purified using the Monarch RNA Cleanup Kit (New England BioLabs, Cat# T2040) according to the manufacturer’s instructions.

Techniques: Modification, Labeling, Expressing, Construct, Western Blot, Control, Plasmid Preparation, Incubation, Illumina Sequencing, Agarose Gel Electrophoresis, Molecular Weight, Conjugation Assay, Fluorescence, Staining, Gel Shift

Journal: Pharmaceuticals

Article Title: Synthesis, Anticancer Screening, and In Silico Evaluations of Thieno[2,3- c ]pyridine Derivatives as Hsp90 Inhibitors

doi: 10.3390/ph18020153

Figure Lengend Snippet: HSC3 and RKO cells exhibit positive Annexin V staining with control and after treatment with compound 6i .

Article Snippet: HSC3 and RKO cells were seeded in 6-well plates for 24 h (1 × 10 5 cells/well) and treated with the IC 50 concentrations of compound 6i or DMSO as a vehicle control for 72 h. Floating dead cells and the attached cells were collected, washed with PBS, and resuspended in 100 μL of Annexin V solution for 30 min according to the manufacturer’s instruction (Annexin V-FITC Kit, Miltenyi Biotec, Gaithersburg, MD, USA, Catalog Number 130-092-052) [ ].

Techniques: Staining, Control

Journal: Pharmaceuticals

Article Title: Synthesis, Anticancer Screening, and In Silico Evaluations of Thieno[2,3- c ]pyridine Derivatives as Hsp90 Inhibitors

doi: 10.3390/ph18020153

Figure Lengend Snippet: ( a ) HSC3 control, ( b ) HSC3 treated by the compound 6i , ( c ) RKO control, and ( d ) RKO treated by the compound 6i . X - and Y -axis indicate Annexin V and PI fluorescence intensity, respectively. Numbers in the right upper and right lower quadrants denote percentages of late and early apoptotic cells, respectively (Annexin V positive).

Article Snippet: HSC3 and RKO cells were seeded in 6-well plates for 24 h (1 × 10 5 cells/well) and treated with the IC 50 concentrations of compound 6i or DMSO as a vehicle control for 72 h. Floating dead cells and the attached cells were collected, washed with PBS, and resuspended in 100 μL of Annexin V solution for 30 min according to the manufacturer’s instruction (Annexin V-FITC Kit, Miltenyi Biotec, Gaithersburg, MD, USA, Catalog Number 130-092-052) [ ].

Techniques: Control, Fluorescence

Journal: Cell reports

Article Title: Dual Processing of R-Loops and Topoisomerase I Induces Transcription-Dependent DNA Double-Strand Breaks

doi: 10.1016/j.celrep.2019.08.041

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: SETX (Senataxin) rabbit polyclonal antibody (used for: WB) , Bethyl , Cat# A301-104A; RRID:AB_873128.

Techniques: Recombinant, Transfection, Viability Assay, Imaging, Luciferase, Control, Software, Fluorescence, Microscopy

Journal: Cell reports

Article Title: Dual Processing of R-Loops and Topoisomerase I Induces Transcription-Dependent DNA Double-Strand Breaks

doi: 10.1016/j.celrep.2019.08.041

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CtIP (RBBP8) rabbit polyclonal antibody (used for: WB) , Bethyl , Cat# A300-488A; RRID:AB_2175262.

Techniques: Recombinant, Transfection, Viability Assay, Imaging, Luciferase, Control, Software, Fluorescence, Microscopy